BBS OER Lab Manual

BBS OER Lab Manual

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BBS Module 3 Western blotting - the transfer 1 of 24

duration 5 minutes 41 seconds

BBS Module 3 Western blotting - the transfer

BBS Module 3 Western blotting - the transfer

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Sitting drop vapor diffusion method 2 of 24

duration 11 seconds

Sitting drop vapor diffusion method

Sitting drop vapor diffusion method

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Protein crystals 3 of 24

duration 11 seconds

Protein crystals

Protein crystals

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Workflow - Bradford assay plate setup 4 of 24

duration 21 seconds

Workflow - Bradford assay plate setup

Workflow - Bradford assay plate setup

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Workflow: setting up a BSA standard curve… 5 of 24

duration 35 seconds

Workflow: setting up a BSA standard curve…

Workflow: setting up a BSA standard curve (Bradford Assay)

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Workflow: DHFR purification using Ni-NTA affinity… 6 of 24

duration 1 minute 9 seconds

Workflow: DHFR purification using Ni-NTA affinity…

Workflow: DHFR purification using Ni-NTA affinity chromatography

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Balancing tubes in a microcentrifuge 7 of 24

duration 44 seconds

Balancing tubes in a microcentrifuge

Balancing tubes in a microcentrifuge

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Chapter 3 example calculating ligation ratios 8 of 24

duration 13 minutes 7 seconds

Chapter 3 example calculating ligation ratios

Chapter 3 example calculating ligation ratios

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Pipetting basics 9 of 24

duration 3 minutes 38 seconds

Pipetting basics

Pipetting basics

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Nanodrop 10 of 24

duration 36 seconds

Nanodrop

Nanodrop

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PCR machine 11 of 24

duration 42 seconds

PCR machine

PCR machine

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Making buffers 12 of 24

duration 59 seconds

Making buffers

Making buffers

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DNA agarose gel 13 of 24

duration 2 minutes 22 seconds

DNA agarose gel

DNA agarose gel

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Bacterial transformation (plating cells) 14 of 24

duration 41 seconds

Bacterial transformation (plating cells)

Bacterial transformation (plating cells)

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Proper handling of liquid using pipetman 15 of 24

duration 48 seconds

Proper handling of liquid using pipetman

Proper handling of liquid using pipetman

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BBS OER lab manual welcome video 16 of 24

duration 8 minutes 4 seconds

BBS OER lab manual welcome video

BBS OER lab manual welcome video

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Inoculation, induction, cell lysate preparation 17 of 24

duration 4 minutes 56 seconds

Inoculation, induction, cell lysate preparation

Inoculation, induction, cell lysate preparation

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Crystals observations 18 of 24

duration 2 minutes 33 seconds

Crystals observations

Crystals observations

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Setting up protein crystal trays - sitting drop… 19 of 24

duration 2 minutes 6 seconds

Setting up protein crystal trays - sitting drop…

Setting up protein crystal trays - sitting drop method

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SDS PAGE gels set up 20 of 24

duration 4 minutes 25 seconds

SDS PAGE gels set up

SDS PAGE gels set up

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Plate reader operation 21 of 24

duration 28 seconds

Plate reader operation

Plate reader operation

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Protein purification (manual pack Ni and elution)… 22 of 24

duration 3 minutes 19 seconds

Protein purification (manual pack Ni and elution)…

Protein purification (manual pack Ni and elution) with integration

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Using Multichannel pipette on a 96 wells tray 23 of 24

duration 12 seconds

Using Multichannel pipette on a 96 wells tray

Using Multichannel pipette on a 96 wells tray

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Bradford assay 24 of 24

duration 3 minutes 1 second

Bradford assay

Bradford assay

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Bradford Assay procedure performed in the Teaching Lab. The video contains only background music.

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